By Milton Zaitlin
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Extra info for Biotechnology in Plant Science. Relevance to Agriculture in the Eighties
4. The response of embryogenic celery callus cells to the level of NH^ in the regeneration medium. 5 μΜ kinetin for 21 days. 87o agar solidified medium containing various levels of (NH^)^ S0^. Somatic embryos were counted after 21 days on regeneration medium. Several groups have found that amino acids will stimulate somatic embryogenesis in place of or in combination with ammonium ion (Kamada and Harada, 1971b; Stuart and Strickland, 1984a). In our previous work with alfalfa we found that proline, alanine, glutamine, or arginine will stimulate somatic embryo formation beyond the level achieved by ammonium alone.
4). 5 mM ammonium. We generally supply 5 mM to avoid the preciptious inhibition apparent at higher levels of NH^ . It should be noted that many of the standard plant tissue culture media supply higher or lower amounts of NH, than is optimal for celery regen eration (Gamborg ejt a_l. , 1976;. Somatic Embryos of Alfalfa and Celery 41 AMMONIUM (mM) Fig. 4. The response of embryogenic celery callus cells to the level of NH^ in the regeneration medium. 5 μΜ kinetin for 21 days. 87o agar solidified medium containing various levels of (NH^)^ S0^.
It is important to stress that throughout the above sequence of pollen development the grains are arranged radially in the interior of the cylindrical anther loculus with the single pore of each grain adpressed to the tapetum (Fig. 2c-e). This arrange- Embryogenesis from Pollen In Vitro 53 Fig. 1. Sequence of pollen development in Zea mays cv. A188; al] grains are stained with a ce t oc a rm i ne . (a) Tetrad. (b) Iso lated microspore after dissolution of callose around tetrad. (c) Partially vacuolate microspore with wall and pore clearly visi ble.
Biotechnology in Plant Science. Relevance to Agriculture in the Eighties by Milton Zaitlin