By Willis A. Wood
The seriously acclaimed laboratory typical, Methods in Enzymology, is without doubt one of the such a lot hugely revered courses within the box of biochemistry. considering 1955, every one quantity has been eagerly awaited, usually consulted, and praised by way of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate this present day - really a vital booklet for researchers in all fields of lifestyles sciences.
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Additional resources for Carbohydrate Metabolism, Part C
The table shows the results of a typical purification. Comparable results have been obtained in more than 20 preparations, with overall recoveries ranging from 50 to 75% and final specific activities of 60-66 units/mg. The purified enzyme has been found to be homogeneous when examined by isoelectric focusing and by ultracentrifugal, electrophoretic, and immunological techniques. Properties of Rat Brain Hexokinase The molecular weight determined by the sedimentation equilibrium method was 97,500 --_+500.
Starch Gel Electrophoresis Principle. Starch gel electrophoresis has revealed most clearly that ATP:hexose phosphotransferase activity consists of a number of enzymes depending on the tissue and animal. Glucokinase activity is visualized on starch gels by coupling the typical glueose-6-phosphate dehydrogenase method described above with a color-generating oxidation-reduction dye system. Procedure. Vertical starch gel electrophoresis 14 was carried out at 0-3 ° for 16 hr with a constant current of 6 V / c m across the gel.
Schwartz and R. E. Basford, Biochemistry 6, 1070 (1967). , when there is negligible "absorbance" at wavelengths longer than 320 nm. 11H. Paulus, Anal. Biochem. 32, 91 (1969). 12j. E. Wilson, Arch. Biochem. Biophys. 159, 543 (1973). 18A. C. Chou and J. E. Wilson, Arch. Biochem. , in press.  GLUCOKINASE FROM YEAST 25 process 14 raise the possibility that loss of enzyme-bound phospholipid during the purification may account for the inability of the purified enzyme to bind. 1~J. E. Wilson, Arch.
Carbohydrate Metabolism, Part C by Willis A. Wood