By Jennifer E. Van Eyk, Michael J. Dunn
With its designated description of membrane protein expression, high-throughput and genomic-scale expression experiences, either at the analytical and the preparative scale, this e-book covers the most recent advances within the box. The step by step protocols and sensible examples given for every technique represent sensible suggestion for newbies and specialists alike.
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Extra resources for Cell-free Protein Synthesis: Methods and Protocols
1 ng of protein per spot . This method, although sensitive, does pose serious problems when used in expression analysis studies. Silver stain is known to be nonstoichiometric in that very high abundant protein spots are prone to negative staining effects and appear as holes in the gel; expression analysis on such proteins is virtually impossible. Glutaraldehyde used in sensitizing the gel prior to silver staining is also incompatible with MS analysis and therefore must be removed from the staining protocol or from picked gel plugs before peptide digestion .
Berglund, G. and Lilja, H. (2006) Reproducibility and accuracy of measurements of free and total prostate-speciﬁc antigen in serum vs plasma after long-term storage at À20 8C. Clinical Chemistry, 52, 235–239. , Burkhard, P. , Hochstrasser, D. F. and Sanchez, J. C. (2005) Truncated cystatin C in cerebrospinal ﬂuid: technical artifact or biological process?. Proteomics, 5, 3060–3065. Sanchez, J. , Corthals, G. , Burkhard, P. R. and Hochstrasser, D. F. (2004) Cystatin C as a potential cerebrospinal ﬂuid marker for the diagnosis of Creutzfeldt-Jakob disease.
University Ave Ann Arbor, MI 48109-1055 USA Catherine G. Zimmermann-Ivol Biomedical Proteomics Research Group, Department of Structural Biology and Bioinformatics, Geneva University, 1 Michel Servet, 1211 Geneva Switzerland XXXIII 1 I Technologies Clinical Proteomics. From Diagnosis to Therapy. J. J. ) Copyright ß 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim ISBN: 978-3-527-31637-3 3 1 Preanalytical Issues in Clinical Proteomic Studies Roisean E. Ferguson, Rosamonde E. 1 Introduction Proteomic initiatives have a great potential to contribute to medicine in terms of biomarker or therapeutic target discovery.
Cell-free Protein Synthesis: Methods and Protocols by Jennifer E. Van Eyk, Michael J. Dunn