Electrochemistry II by Craig E. Lunte, William R. Heineman (auth.), E. Steckhan PDF

By Craig E. Lunte, William R. Heineman (auth.), E. Steckhan (eds.)

ISBN-10: 0387182268

ISBN-13: 9780387182261

ISBN-10: 3540182268

ISBN-13: 9783540182269

Contents/Information: C.E. Lunte, W.R. Heineman, Cincinnati, OH, united states: Electrochemical ideas in Bioanalysis

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Phenylalanine hydroxylase activity has been determined by electrochemically monitoring the conversion of tetrahydrobiopterin to dihydrobiopterin 273). Another monooxygenase, tyrosine hydroxylase, has been determined by detecting the DOPA produced by the enzymatic reaction 274, 275). Formation of DOPA has also been monitored electrochemically to determine the activity of L-aromatic amino acid decarboxylase 276). Other enzymes involved in catecholamine metabolism which have been determined electrochemically include: dopamine-13-hydroxylase 277~, phenylethanolamine-N-methyltransferase 278~, and catechol-O-methyltransferase 279~.

Montalvo, J. : J. Am. Chem. Soc. 91, 2164 (1969) 45. Guilbault, G. , Montalvo, J. G. : ibid. 92, 2533 (1970) 46. Guilbault, G. , Montalvo, J. G. : Anal. Lett. 2, 283 (1969) 47. : C. R. Acad. Sci. Paris Ser. C275, 309 (1972) 48. Guilbault, G. , Nagy, G. : Anal. Chem. 45, 4t7 (1973) 49. , Graneli, A , Jagner, D. : Anal. Lett. 6, 969 (1973) 50. Guilbault, G. : Anal. Chim. Acta 73, 355 (1974) 51. Papastathopoulos, D. , Rechnitz, G. A. : ibid. 79, 17 (1975) 52. , Guilbault, G. G. : Anal. Chem. 49, 795 (1977) 53.

The limited selectivity of in vivo electrochemical techniques is also a disadvantage at present. However, the ability to make rapid measurements for continuous sampling to follow the dynamics of neurochemical processes more than offsets the disadvantages of the technique. It cannot be over emphasized that at present only in vivo electrochemical measurements provide a method of monitoring rapid changes in neurotransmitter concentration. Another advantage of in vivo electrochemical techniques is that tissue damage is less severe than with alternative perfusion techniques.

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Electrochemistry II by Craig E. Lunte, William R. Heineman (auth.), E. Steckhan (eds.)


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