Download e-book for iPad: Hybridoma Technology in the Biosciences and Medicine by Zelig Eshhar (auth.), Timothy A. Springer (eds.)

By Zelig Eshhar (auth.), Timothy A. Springer (eds.)

ISBN-10: 1468449648

ISBN-13: 9781468449648

ISBN-10: 1468449664

ISBN-13: 9781468449662

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Extra resources for Hybridoma Technology in the Biosciences and Medicine

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57 For IgM antibodies that are advantageous for complement-mediated lysis, agglutination, or precipitation of polymeric antigens, primary responses are preferable. g of soluble antigen is given intravenously, and 3-4 days later the spleen cells are taken for fusion. 58 Primary responses to particulate antigens (red cells or viruses)59 and secondary responses to soluble antigens can also be elicited in vitro. 50-52 In this case, normal spleen cells or immune spleen cells are incubated with the antigen for 4-6 days, and then are used for fusion.

In molecular terms, class switching results from a second kind of DNA rearrangement that joins the V gene in front of different constant genes. 36 ,37 However, the cellular events that are involved in class switching are not yet well defined, and it is not yet known whether this process is internally programmed or externally induced. Some of the B cells (before and after class switching) do not differentiate into the terminal stage of antibody-secreting plasma cells. Such cells cease to divide and become memory cells.

58 Primary responses to particulate antigens (red cells or viruses)59 and secondary responses to soluble antigens can also be elicited in vitro. 50-52 In this case, normal spleen cells or immune spleen cells are incubated with the antigen for 4-6 days, and then are used for fusion. Most groups use T-cell-conditioned medium to support and facilitate the stimulation of the antibody-forming cells. Although it has been claimed that in vitro immunization requires less antigen and is more efficient, it has not yet justified itself as replacement for the in vivo immunization, and might be tried as a method to boost B cells when weak response is observed in the immunized animals.

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Hybridoma Technology in the Biosciences and Medicine by Zelig Eshhar (auth.), Timothy A. Springer (eds.)


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