New PDF release: Laboratory Techniques In Microbiology & Biotechnology

By Tiwari, G. S. Hoondal

ISBN-10: 8182470773

ISBN-13: 9788182470774

Show description

Read Online or Download Laboratory Techniques In Microbiology & Biotechnology PDF

Similar biotechnology books

Read e-book online Environmental Biotechnology Principles and Applications PDF

In Environmental Biotechnology-Principles and functions, the authors attach the numerous varied features of environmental biotechnology. The ebook develops the elemental innovations and quantitative instruments within the first six chapters, which include the foundations. The textual content regularly calls upon these rules because it describes the purposes in Chapters 7 via sixteen.

Get Industrial Application of Immobilized Biocatalysts PDF

Bargains sensible examples of bioreactor structures that use immobilized biocatalysts - together with enzymes and microbial cells - which were carried out at the commercial point in Japan and Denmark. The publication offers info at the present prestige of profitable new bioreactor applied sciences.

Download e-book for iPad: Biotechnology of Crucifers by Surinder Kumar Gupta Ph.D. (auth.), Surinder Kumar Gupta

Regardless of the hot advances made within the development of crucifer vegetation utilizing traditional breeding suggestions, the yield degrees and the oil and meal caliber couldn't be more desirable as anticipated. the certainty of genetic fabric (DNA/RNA) and its manipulation through scientists has supplied the chance to enhance crucifers by way of expanding its range past traditional genetic barriers.

Download e-book for iPad: Multifunctional Molecular Materials by Lahcene Ouahab

This ebook presents a finished evaluate on multifunctional molecular fabrics that contain coexistence or interaction or synergy among a number of actual homes targeting electric conductivity, magnetism, single-molecule magnets habit, chirality, spin crossover, and luminescence. The book’s insurance levels from transition metals and lanthanide coordination complexes to real natural fabrics.

Additional resources for Laboratory Techniques In Microbiology & Biotechnology

Example text

2. Heat fix the smear and treat the smear for 10 min. with methylene blue stain. 3. Wash the slide under running tap water and stain the smear with carbol fuchsin for a shol1 while (5 sec) and wash immediately with water. 4. Blot dry and examine under oil immersion objective. Live vegetative cells appear purple and dead are stained red or pink. Live spores take up faint pink color and dead take up hilic color. Questions I. What is the concentration of methylene 'blue or carbol fuchsin? 2. What is intravital stain?

Iii)Neutral red solution: for modified Neisser method - Mix 1 g neutral red and 2 ml 1% glacial acetic acid in 1000 ml distilled water. Loeffler method Loeffler's methylene blue stain Procedure Albert method I. Prepare bacterial smear on clean grease free slide, air dry and heat fix it. 2. Stain it with Albert stain for 5 min. 3. Wash the stain with running tap water. Add Albert's iodine for Imin. 4. Wash with water, blot dry, and examine under oil immersion objective. Record the observations.

Now add more Ag NO, solution drop wise until precipitates returns and does not dissolve) Mordant (phenol I g and tannic acid 5g dissolved in 100 ml distilled water) Becker's method a. b. Fixative and mordant same as above. Stain: Basic fuchsin saturated alcoholic solution 45 ml mixed with 18 ml Shunk's mordant (spirit 18 ml and aniline oil 4 ml) and volume made to 100 ml with distilled water. Procedure Fontana's method I. 2. 3. 4. 5. 6. 7. Scrap material from the teeth using sterile needle. Transfer the material to slide and spread this on to the slide to prepare a thin smear.

Download PDF sample

Laboratory Techniques In Microbiology & Biotechnology by Tiwari, G. S. Hoondal


by Kenneth
4.1

Rated 4.20 of 5 – based on 35 votes