By Seymour Kaufman
The significantly acclaimed laboratory usual, Methods in Enzymology, is likely one of the so much hugely revered courses within the box of biochemistry. due to the fact 1955, each one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate this day - actually a necessary booklet for researchers in all fields of existence sciences
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Additional info for Metabolism of Aromatic Amino Acids and Amines
Shiman, J. Biol. Chem. 257, 845 (1982). ~5 R. Shiman and L. S. Jefferson, J. Biol. Chem. 257, 839 (1982). 15 Other than the difference in specific activity, iron-treated and -untreated enzymes behave exactly the same in our purification procedures. Procedures Unless otherwise stated all procedures were performed at 0-4 °, buffer pH was determined at 0 °, and all solutions were made with deionized, glass-distilled water. IA. Preparation of Rat Liver Extract. Minced fresh rat livers are homogenized in 4 ml of homogenization buffer/g of liver.
6 has lost the phosphorylating site, perhaps by limited proteolysis at the N-terminal end of the protein. The study of the amino acid sequence of the human enzyme deduced from the cDNA clone t4 has revealed that the amino acid sequence at residues 13-21 of the amino-terminus is identical to the amino acid sequence which comprise the phosphorylating site in the rat enzyme~6: P I Arg-Lys-Leu-Ser-Asx-Phe-Gly-Glx-Glx 16 M. Wretborn, E. Humble, U. Ragnarsson, and L. Engstr6m, Biochem. Biophys. Res. Comrnun.
D. Ledley, A. G. DiLella, K. J. H. Robson, and S. L. C. Woo, Biochemistry 24, 556 (1985). 9 ND 30 13 24 40 24 28 56 23 25 28 9 19 2 28 50 22 27 3 From Abita et a l : The numbers of residues are based on a subunit molecular weight of 55,000. ~4 a lase deduced from the nucleotide sequence of a full-length cDNA clone. This amino acid sequence constitutes a protein of 51,672 Da composed of 451 amino acid residues. However, this predicted amino acid composition differs somewhat from that reported by Abita e t al.
Metabolism of Aromatic Amino Acids and Amines by Seymour Kaufman