By Sarker, Latif & Gray
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Extra info for Methods In Biotechnology Vol 20 Natural Products Isolation
This allows a relatively good set-up to monitor both the growth rate of the biomass and the production of metabolites. It also provides a means of carrying out initial studies to optimize culture conditions and increase metabolite production (see Note 15). When performing studies in a small fermenter, the culture is grown under controlled conditions (see Note 16). , aeration, stirring speed, temperature, pH, oxygen, and carbonic acid concentration), and the absence of external contamination has been ascertained.
Dilutions are made to obtain concentrations of 5Â10À2, 5 Â 10À3, 5 Â 10 À 4, 5 Â 10À5, 5 Â 10À6, 5 Â 10À7, 5 Â 10À8, 5Â10À9, 5 Â 10À10 mg/mL. 00 mL) and allowed to stand for 30 min for any reaction to take place. The UV absorbance of these solutions is recorded at 517 nm. The experiment is usually performed in triplicate and the average absorption is noted for each concentration. The same procedure is followed for the standard (quercetin). 2. Antibacterial Serial Dilution Assay Using Resazurin as an Indicator of Cell Growth Antibacterial activity of extracts, fractions, or puriﬁed compounds can be assessed and the minimal inhibitory concentration (MIC) value determined by this assay (18,19).
Initial and Bulk Extraction 41 4. Notes 1. Several databases and journals (such as the Journal of Ethnopharmacology) containing some ethnobotanical, chemical, and biological information can be consulted on the Internet. htm 2. , methanol or ethanol). 3. , the volatile constituents of essential oils). 4. Solvent removal should be done immediately after extraction to minimize the loss of compounds unstable in solution. Prolonged exposure to sunlight should also be avoided because of the potential for degradation.
Methods In Biotechnology Vol 20 Natural Products Isolation by Sarker, Latif & Gray