New PDF release: Nano-Bio Probe Design and Its Application for Biochemical

By Bang-Ce Ye, Min Zhang, Bin-Cheng Yin

ISBN-10: 3642295428

ISBN-13: 9783642295423

ISBN-10: 3642295436

ISBN-13: 9783642295430

In this quantity, Prof. Ye and his coworkers suggest and evaluation the concept that of nano-bio probe layout for biochemical research at the foundation in their contemporary released works. a special biochemical research expertise according to fluorescence polarization superior through nanoparticles is defined the following with profitable purposes in environmental tracking, quick and delicate sensing protease task and high-throughput screening of inhibitors. additionally, they introduce a flexible molecular beacon (MB)-like explore for the multiplex sensing of objectives equivalent to sequence-specific DNA, protein, steel ions and small molecule compounds in response to the self-assembled biomolecule-graphene conjugates. along with, a few colorimetric and luminescence probes using steel nanoparticles for biochemical purposes also are awarded, equivalent to chiral enantiomer discrimination and separation, environmental tracking, sanatorium analysis and etc.

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1021/ja076716c 34. Ozkan M, Kim JH, Chaudhary S (2007) Multicolour hybrid nanoprobes of molecular beacon conjugated quantum dots: FRET and gel electrophoresis assisted target DNA detection. Nanotechnology 18(19):195105. 1088/0957-4484/18/19/195105 35. Swathi RS, Sebastian KL (2009) Long range resonance energy transfer from a dye molecule to graphene has (distance)(-4) dependence. J Chem Phys 130(8):086101. 3077292 36. Chang H, Tang L, Wang Y, Jiang J, Li J (2010) Graphene fluorescence resonance energy transfer aptasensor for the thrombin detection.

Angew Chem Int Ed Engl 49(45):8454–8457. 201002822 References 37 40. Wang Y, Li Z, Hu D, Lin CT, Li J, Lin Y (2010) Aptamer/graphene oxide nanocomplex for in situ molecular probing in living cells. J Am Chem Soc 132(27):9274–9276. 1021/ ja103169v 41. Pu Y, Zhu Z, Han D, Liu H, Liu J, Liao J, Zhang K, Tan W (2011) Insulin-binding aptamerconjugated graphene oxide for insulin detection. Analyst 136(20):4138–4140. 1039/ c1an15407a 42. Preethi BL, Jaisri G, Kumar KM, Sharma R (2011) Assessment of insulin resistance in normoglycemic young adults.

By monitoring the increase in fluorescence intensity, the target ATP could be detected with very high sensitivity [39]. Moreover, Wang et al. designed amplified ATP assay platform for sensitive ATP detection in vivo [40]. Also, similar to Yang’s group, Tan’s group reported a simple and sensitive aptamer/GO based assay for insulin detection with high sensitivity via the catalytic recycling of the analyte [41]. The detection of insulin is especially significant in clinical work, because the level of insulin is the most critical indicator of the endocrinal function of beta cells and serves as a valuable basis for the diagnosis of diabetes mellitus, insulinoma, insulin resistant syndrome, etc.

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Nano-Bio Probe Design and Its Application for Biochemical Analysis by Bang-Ce Ye, Min Zhang, Bin-Cheng Yin

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