By Michal Janitz
Written through best specialists from and academia, this primary unmarried entire source addresses fresh advancements in subsequent iteration DNA sequencing expertise and their impression on genome study, drug discovery and healthiness care. As such, it provides an in depth comparative research of commercially to be had structures in addition to insights into substitute, rising sequencing suggestions. additionally, the e-book not just covers the rules of DNA sequencing recommendations but additionally social, moral and advertisement features, the concept that of custom-made drugs and a five-year viewpoint of DNA sequencing.Content:
Chapter 1 Sanger DNA Sequencing (pages 1–11): Artem E. males, Peter Wilson, Kirby Siemering and Susan Forrest
Chapter 2 Illumina Genome Analyzer II procedure (pages 13–28): Abizar Lakdawalla and Harper Vansteenhouse
Chapter three utilized Biosystems stable™ approach: Ligation?Based Sequencing (pages 29–42): Vicki Pandey, Robert C. Nutter and Ellen Prediger
Chapter four The Next?Generation Genome Sequencing: 454/Roche GS FLX (pages 43–56): Lei Du and Michael Egholm
Chapter five Polony Sequencing: heritage, know-how, and purposes (pages 57–76): Jeremy S. Edwards
Chapter 6 Next?Generation series information research (pages 77–88): Leonard N. Bloksberg
Chapter 7 DNASTAR's Next?Generation software program (pages 89–94): Tim Durfee and Thomas E. Schwei
Chapter eight Real?Time DNA Sequencing (pages 95–101): Susan H. Hardin
Chapter nine Direct Sequencing by means of TEM of Z?Substituted DNA Molecules (pages 103–116): William ok. Thomas and William Glover
Chapter 10 A unmarried DNA Molecule Barcoding process with purposes in DNA Mapping and Molecular Haplotyping (pages 117–132): Ming Xiao and Pui?Yan Kwok
Chapter eleven Optical Sequencing: Acquisition from Mapped Single?Molecule Templates (pages 133–151): Shiguo Zhou, Louise Pape and David C. Schwartz
Chapter 12 Microchip?Based Sanger Sequencing of DNA (pages 153–163): Ryan E. Forster, Christopher P. Fredlake and Annelise E. Barron
Chapter thirteen Multiplex Sequencing of Paired finish Ditags for Transcriptome and Genome research (pages 165–182): Chia?Lin Wei and Yijun Ruan
Chapter 14 Paleogenomics utilizing the 454 Sequencing Platform (pages 183–199): M. Thomas P. Gilbert
Chapter 15 ChIP?Seq: Mapping of Protein–DNA Interactions (pages 201–215): Anthony Peter Fejes and Steven J. M. Jones
Chapter sixteen MicroRNA Discovery and Expression Profiling utilizing Next?Generation Sequencing (pages 217–228): Eugene Berezikov and Edwin Cuppen
Chapter 17 DeepSAGE: Tag?Based Transcriptome research past Microarrays (pages 229–244): Kare L. Nielsen, Annabeth H. Petersen and Jeppe Emmersen
Chapter 18 the hot Genomics and private Genome details: moral matters (pages 245–254): Jeantine E. Lunshof
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Additional resources for Next Generation Genome Sequencing: Towards Personalized Medicine
This combination produces a highly robust system with the accuracy necessary to support high-throughput variation detection. Several additional features – probe speciﬁcity, highly efﬁcient chemical cleavage of the ligated probes, and dye-labeled sequencing products – further differentiate the technology of the SOLiD System from that of its competitors, rendering it unique as a platform for high-throughput discovery. Next-Generation Genome Sequencing: Towards Personalized Medicine. Edited by Michal Janitz Copyright Ó 2008 WILEY-VCH Verlag GmbH & Co.
Developed specifically for tag-based sequencing applications on the Genome Analyzer, GenomeStudio presents genome-scale data in the form of user-friendly graphical views with the ability to zoom in from the level of chromosomes to a single base. The y-axis of the graph shows the number of reads mapping to base positions (x-axis) for a library prepared from a chromatin immunoprecipitation experiment. 5 Copy number variation in the human X-chromosome. Paired end sequence data were generated from an isolated Caucasian X-chromosome according to standard Genome Analyzer protocols.
Recently, Applied Biosystems developed the SOLiD Small RNA Expression Kit which converts, in a single day, a few nanograms of RNA into cDNA libraries. The sequence of these tags is used to study expression levels of individual miRNAs and discover novel ncRNAs. Experiments using this kit have already shown that the organization of miRNAs is very complex. Novel miRNAs have been predicted based on known miRNA structure and these miRNAs are currently being validated. Additionally, the presence of miRNA isoforms (isomiRs, RNA molecules having 50 and 30 ends that differ from the reference sequence) have been shown to be present, thereby complicating our understanding of the role that these molecules play in controlling gene expression.
Next Generation Genome Sequencing: Towards Personalized Medicine by Michal Janitz