Nucleic Acids from A to Z: A Concise Encyclopedia - download pdf or read online

By Sabine Müller

ISBN-10: 3527312110

ISBN-13: 9783527312115

ISBN-10: 3527622527

ISBN-13: 9783527622528

Concise yet entire, this mini-encyclopedia includes over 1,500 entries masking all vital recommendations, compounds, concepts and acronyms for speedy and simple reference.
Guiding readers in the course of the ever-increasing jungle of nucleic acid technological know-how and know-how, the e-book distills the major info out of the big physique of basic literature and provides it in one volume.
A first-stop source for everybody, from scholars to tested researchers, as either a computer and library reference.Content:

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Additional info for Nucleic Acids from A to Z: A Concise Encyclopedia

Example text

The ability to resolve protein–DNA complexes depends largely upon the stability of the complex during the brief time it is migrating into the gel. Sequence-specific interactions are transient and are stabilized by the relatively low ionic strength of the * electrophoresis buffer used. Upon entry into the gel, protein complexes are quickly band shifting Observation of certain nucleic acid species migrating as a band in a gel during * gel electrophoresis (→band shift assay). Classically, the binding of * DNA-binding proteins has been the majority of cases in which band shifts were observed originally.

A * RNA library is mixed and incubated with a substance that might attach itself covalently to the RNA. RNA molecules that accelerate such a tagging reaction are then physically separated from the unmodified members of the RNA library, enzymatically amplified, and subjected to further rounds of selection and amplification. By tethering a second substrate to the RNA pool members, it has become possible to expand the scope of RNA catalysis to reactions between two small organic molecules, like the Diels–Alder and Aldol reactions.

From the integral of this peak the enthalpy of * melting and from its onset the * melting temperature can be determined. In ITC the heat of reaction as it is taken up or released during a biomolecular interaction is monitored. If * ligand is titrated to a receptor molecule, the measured heat Caged molecules, in general, are used to investigate the biology of receptors, ion channels, neuronal activation sites or the mode of action of enzymes, peptides and nucleic acids (extensively reviewed in G.

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Nucleic Acids from A to Z: A Concise Encyclopedia by Sabine Müller

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