By John Whittall, Peter W. Sutton
Biocatalysts are more and more utilized by chemists engaged in positive chemical synthesis inside of either and academia. at the present time, there exists an immense number of high-tech enzymes and entire mobilephone biocatalysts, which upload significantly to the repertoire of man-made percentages.
Practical equipment for Biocatalysis and Biotransformations 2 is a "how-to" advisor that makes a speciality of the sensible purposes of enzymes and lines of microorganisms which are with no trouble acquired or derived from tradition collections. The resources of beginning fabrics and reagents, tricks, advice and protection recommendation (where acceptable) are given to make sure, so far as attainable, that the strategies are reproducible. Comparisons to substitute technique are given and proper references to the first literature are stated. This moment quantity – which are used by itself or together with the 1st quantity - concentrates on new functions and new enzyme households stated because the first quantity. Contents include:
- introduction to contemporary advancements and destiny wishes in biocatalysts and artificial biology in industry
- reductive amination
- enoate reductases for relief of electron poor alkenes
- industrial carbonyl reduction
- regio- and stereo- selective hydroxylation
- oxidation of alcohols
- selective oxidation
- industrial hydrolases and similar enzymes
- transferases for alkylation, glycosylation and phosphorylation
- C-C bond formation and decarboxylation
- halogenation/dehalogenation/heteroatom oxidation
- tandem and sequential multi-enzymatic syntheses
Practical equipment for Biocatalysis and Biotransformations 2 is a vital number of biocatalytic tools for chemical synthesis in an effort to discover a position at the bookshelves of man-made natural chemists, pharmaceutical chemists, and strategy R&D chemists in and academia.
Chapter 1 Biocatalysis within the high-quality Chemical and Pharmaceutical Industries (pages 1–59): Peter W. Sutton, Joseph P. Adams, Ian Archer, Daniel Auriol, Manuela Avi, Cecilia Branneby, Andrew J. Collis, Bruno Dumas, Thomas Eckrich, Ian Fotheringham, Rob ter Halle, Steven Hanlon, Marvin Hansen, okay. E. Holt?Tiffin, Roger M. Howard, Gjalt W. Huisman, Hans Iding, Kurt Kiewel, Matthias Kittelmann, Ernst Kupfer, Kurt Laumen, Fabrice Lefevre, Stephan Luetz, David P. Mangan, Van A. Martin, Hans?Peter Meyer, Thomas S. Moody, Antonio Osorio?Lozada, Karen Robins, Radka Snajdrova, Matthew D. Truppo, Andrew Wells, Beat Wirz and John W. Wong
Chapter 2 Reductive Amination (pages 61–86): Simon Willies, Matthew D. Truppo, Christopher ok. Savile, Jacob M. Janey, Jeffrey C. Moore, Gjalt W. Huisman, Gregory J. Hughes, Francesco G. Mutti, Christine S. Fuchs, Wolfgang Kroutil, Jennifer Hopwood, Matthew D. Truppo, Richard Lloyd, Nicholas J. Turner, Eun younger Hong, Minho Cha, Hyungdon Yun, Byung?Gee Kim, S. Stella and Anju Chadha
Chapter three Enoate Reductases for aid of Electron poor Alkenes (pages 87–114): Melanie corridor, Christoph okay. Winkler, Gabor Tasnadi, Kurt Faber, Elisabetta Brenna, Francesco G. Gatti, Fabio Parmeggiani, Pietro Buzzini, Marta Goretti, Chiara Ponzoni, Elisa Caselli, Eva Branda, Maria Rita Cramarossa, Benedetta Turchetti, Luca Forti, Adam Z. Walton, Bradford T. Sullivan, Jon D. Stewart, Maximiliano A. Sortino and Susana A. Zacchino
Chapter four commercial Carbonyl aid (pages 115–127): David P. Mangan, Thomas S. Moody, Owen Gooding, Gjalt W. Huisman, Carlos A. Martinez, Adam Smogowicz, Jeremy S. Steflik, Maria S. Brown, Katarina S. Midelfort, Michael P. Burns, John W. Wong and Jack Liang
Chapter five Regio? and Stereoselective Hydroxylation (pages 129–162): Marcus Hans, Jan?Metske van der Laan, Ben Meijrink, Wibo van Scheppingen, Richard Kerkman, Marco van den Berg, Matthias Kittelmann, Anton Kuhn, Annina Riepp, Jurgen Kuhnol, Andreas Fredenhagen, Lukas Oberer, Oreste Ghisalba, Stephan Luetz, David P. Mangan, Thomas S. Moody, David Schmid, Antonio Osorio?Lozada, F. Ozde. Utkur, Jonathan Collins, Christoph Brandenbusch, Gabriele Sadowski, Andreas Schmid, Bruno Buhler, Matthias Kinne, Marzena Poraj?Kobielska, Rene Ullrich, Martin Hofrichter, Gideon Grogan and Mark L. Thompson
Chapter 6 Oxidation of Alcohols (pages 163–179): Rajesh Kumar, Jim Cawley, Michael Karmilowicz, Carlos A. Martinez, Nathan Wymer, Bo Yuan, Franck Escalettes, Nicholas J. Turner, Johann H. Sattler, Katharina Tauber, Francesco G. Mutti and Wolfgang Kroutil
Chapter 7 Selective Oxidation (pages 181–202): Valentin Kohler, Bas Groenendaal, Kevin R. Bailey, Anass Znabet, James Raferty, Madeleine Helliwell, Nicholas J. Turner, Kristian Geitner, Uwe T. Bornscheuer, Gonzalo de Gonzalo, Vicente Gotor, Shuvendu Das, John P. N. Rosazza, Aashrita Rajagopalan, Francesco G. Mutti and Wolfgang Kroutil
Chapter eight business Hydrolases and comparable Enzymes (pages 203–229): Mark Dow, Rebecca Meadows, Rhona Sinclair, Andrew Wells, Gary Breen, John Carey, Fiona Rawlinson, okay. E. Holt?tiffin, M. C. Lloyd, Beat Wirz, Paul Spurr, Christophe Pfleger, Graham Checksfield, Stewart T. Hayes, Wilfried Hoffmann, Christian T. Regius, Gemma Scotney, Sarah L. Spencer, Roger M. Howard, Colin M. Burns, Gordon B. Ward, Robert Walton, Neil Barnwell, Matthew D. Truppo and Gregory J. Hughes
Chapter nine Transferases for Alkylation, Glycosylation and Phosphorylation (pages 231–262): Daniel Auriol, Rob ter Halle, Fabrice Lefevre, Daniel F. Visser, Gregory E. R. Gordon, Moira L. Bode, Kgama Mathiba, Dean Brady, Karel De wintry weather, Tom Desmet, An Cerdobbel, Wim Soetaert, Teunie van Herk, Aloysius F. Hartog, Ron Wever, Malgorzata Brzezinska?rodak, Magdalena Klimek?Ochab, Ewa Zymanczyk?Duda, Joyeeta Mukherjee, Munishwar N. Gupta, Wen?Bing Yin, Shu?Ming Li and Mandana Gruber?Khadjawi
Chapter 10 C–C Bond Formation and Decarboxylation (pages 263–295): Alessandra Bonamore, Alberto Macone, Wolfgang Kroutil, Birgit Seisser, Tijs M. Lammens, Daniela De Biase, Maurice C. R. Franssen, Elinor L. Scott, Johan P. M. Sanders, Kenji Miyamoto, Robert Kourist, Shosuke Yoshida, Hiromichi Ohta, Jie Tian, Heng Li, Wen?Yun Gao, Saravanakumar Shanmuganathan, Dessy Natalia, Lasse Greiner, Pablo Dominguez de Maria, Yan?Hong He, Zhi Guan and Jian?Feng Cai
Chapter eleven Halogenation/Dehalogenation/Heteroatom Oxidation (pages 297–312): Jia Zeng, Jixun Zhan, Zbynek Prokop, Veronika Stepankova, Khomaini Hasan, Radka Chaloupkova, Jiri Damborsky, Ai?Tao Li, Gao?Wei Zheng, Hui?Lei Yu, Jiang Pan, Chun?Xiu Li, Jian?He Xu, Marian Mikolajczyk, Jerzy luczak and Piotr Kielbasinski
Chapter 12 Tandem and Sequential Multi?Enzymatic Syntheses (pages 313–345): Bong?Gyu Kim, Joong?Hoon Ahn, Guido Sello, Patrizia Di Gennaro, Teunie van Herk, Aloysius F. Hartog, Ron Wever, Isabel Oroz?Guinea, Israel Sanchez?Moreno, Eduardo Garcia?Junceda, Bian Wu, Wiktor Szymanski, Ben L. Feringa, Dick B. Janssen, Ly Villo, Malle Kreen, Marina Kudryashova, Andrus Metsala, Sven Tamp, ulo Lille, Tonis Pehk, Omar Parve, Kathleen McClean and Peter Eddowes
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Extra info for Practical Methods for Biocatalysis and Biotransformations 2
1). 2). Novozym 435 was included as a reference point for comparison to the standard process. genzyme. 50% higher speciﬁc activity was observed with the CAL-B immobilized on EXE120. Additionally, Novozym 435 retained only 6% of its initial activity after 48 h under process conditions compared to 94% activity retention for the EXE120 immobilized CAL-B (MRK-CALB-EXE120). Novozym 435 and MRK-CALB-EXE120 were then compared head to head in continuous packed bed plug ﬂow reactor mode. 3). Additionally, higher product yield and ee was obtained using the higher speciﬁc activity MRK-CALB-EXE120 compared to Novozym 435 (95% yield vs 90% and 88% ee vs 86%).
2). As the number of suitable advanced projects is comparatively low, activities such as creating tailor-made enzymes by means of directed evolution still remains a rare event (in two projects26, 30 the respective activities had been initiated but were abandoned again when the respective projects were discontinued). In general, outsourcing is considered an important tool extension but 12 Biocatalysis in the Fine Chemical and Pharmaceutical Industries restricted to later phase projects. Examples are enzyme screenings for bulky substrates, the heterologous expression of various enzymes and assay development.
In contrast, a single step, stereo and regioselective synthesis could be performed in the presence of glycosyltransferases, able to transfer the sugar moiety of a sugar nucleotide (UDP-glucose) to an acceptor. 68 Nevertheless, this approach is impaired by the very high cost of the sugar nucleotides which require in situ regeneration. 3 glucoside. 3). 1 for experimental details). The advantages of this biocatalytic route are numerous: . . . Use of cheap sucrose instead of peracylated thioglucosides as glycosylating reagent69 One-step process instead of two steps Perfect regioselectivity at the 4-position without need for protection Perfect stereoselectivity at the anomeric position Use of water as a solvent and no hazardous chemicals These two examples, which are currently running at a scale of 10–100 kg per batch, clearly show that biocatalysis can offer a feasible alternative to chemical synthesis in terms of economics, environmental impact and ease of handling.
Practical Methods for Biocatalysis and Biotransformations 2 by John Whittall, Peter W. Sutton