By H. H. Cole and P. T. Cupps (Eds.)
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Zarrow, M. , in "Sex and Internal Secretions" (W. C. ), 3rd ed.. Vol. 2, p. 986. Williams & Wilkins, Baltimore, Maryland, 1961. 163. , Klin. Wochschr. 6, 248 ( 1 9 2 7 ) . 164. , Nature 139, 112 ( 1 9 3 7 ) . IMMUNOLOGICAL CHARACTERIZATION OF THE GONADOTROPINS^ A. REES MIDGLEY, J R . t I. II. III. IV. Introduction 47 General Immunological Considerations 48 Development of Antisera 49 Immunological Methods of Analysis 50 A. Precipitation of Hormone-Antibody Complexes 50 B. Complement Fixation 51 C.
Williams & Wilkins, Baltimore, Maryland, 1939. 48. , Acta Endocrinol 54, 91 (1967). 49. Evans, H. , Western J. Surg. Obstet. Gynecol March-April (1936). 50. Evans, H. , Pencharz, R. , and Simpson, M. , Univ. Calif. Publ Anat, Berkeley 1, 237 (1936). 51. Evans, H. , Meyer, R. , and Simpson, M. , Mem. Univ. Calif. 11, 151 (1933). 52. Evans, H. , and Simpson, M. , in "The Hormones" (G. Pincus and K. V. ). Vol. 2, pp. 351-404. Academic Press, New York, 1950. 53. Evans, H. , Simpson, M. , and Austin, P.
ASSAY O F PMSG Cole and Erway ( 3 3 ) proposed a 48-hour method of assay based upon the ovarian weight response of 25-day-old immature female rats. A slight modification involves a two-point assay using younger (22-day-old) rats ( 3 1 ) . This procedure is more rapid and simpler than the older 96-hour minimal response assays. It is more specific and probably more accurate than assays based on uterine weight. VII. Effect of Gonoadotropins upon Metabolic Activity of Gonads It is well known that gonadotropins influence directly or indirectly the synthesis of steroid hormones by the gonads and are also responsible for the growth of gonadal tissues.
Reproduction in Domestic Animals by H. H. Cole and P. T. Cupps (Eds.)