Download e-book for iPad: The Evolutionary History and a Systematic Revision of by James L. Patton

By James L. Patton

ISBN-10: 0520098668

ISBN-13: 9780520098664

Information the evolutionary background of the barren region woodrat complicated (lepida staff, genus Neotoma) of western North the US. The analyses contain normal multivariate morphometrics of museum specimens coupled with mitochondrial and nuclear DNA sequences and microsatellite loci. The paintings additionally lines the spatial and temporal diversification of this crew of barren region living rodents, revising species obstacles and delineating subspecies thought of legitimate.

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Additional info for The Evolutionary History and a Systematic Revision of Woodrats of the Neotoma lepida Group (University of California Publications in Zoology)

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Individuals in all five age-categories had adult pelage, although they may not have been post-reproductive. We included all age 1-5 individuals in the analyses we undertook. Exceptions to this “age” 12 University of California Publications in Zoology scheme are the very large-bodied individuals from Baja California, where animals in adult pelage and with evidence of reproductive activity may also have incompletely erupted molars wherein the base of the hypoflexus is below the bony alveolus. These specimens were measured and tested to determine if they could be included in an “adult” category for statistical comparisons.

We generated all sequences on either an ABI 377 slab gel or ABI 3730 capillary automated sequencer following manufacturer protocols. ). Both strands of the entire gene were sequenced for an initial set of 203 specimens to ensure the constancy of sequence for each individual, but only the light strand was then obtained for subsequent specimens, which comprised the majority of the sequences examined. , 2005). In each case, a small piece of skin was removed from the edge of the ventral incision with sterilized instruments, hair was carefully removed by a sterile scalpel blade, the skin fragment was subsequently soaked in sterile ddH20 overnight, with extraction then performed with the DNAeasy kits in the same fashion as tissue samples for freshly collected samples.

Lepida group are identified and are described in greater detail immediately below. 30 University of California Publications in Zoology Both the MP and Bayesian analyses delineated the same series of subclades (Fig. 5), most of which had been previously defined by Patton and Álvarez-Castañeda (2005) and Matocq et al. (2007). These latter two studies differ from the results presented here only in our addition of subclade 1D (the single individual of N. l. insularis from Isla Ángel de la Guarda) and in the slightly different topologies of some subclades within both major clades (detailed below).

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The Evolutionary History and a Systematic Revision of Woodrats of the Neotoma lepida Group (University of California Publications in Zoology) by James L. Patton

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