Download e-book for kindle: The Microflow Cytometer by Frances S. Ligler, Jason S. Kim

By Frances S. Ligler, Jason S. Kim

ISBN-10: 9814267414

ISBN-13: 9789814267410

The box of microfluidic movement cytometry is becoming at a quick cost. This quantity describes the advance of cheap, moveable move cytometers via incorporation of microfluidic applied sciences and small optical parts. The publication discusses the underlying microfluidic theories crucial for microflow cytometry, in addition to advances which are consultant of the present state of the art. a variety of learn teams major the sphere are at the moment providing layout and fabrication innovations for those leading edge part applied sciences, and the ebook reports integration of the parts into useful prototype units for research and manipulation of debris and cells. It additionally examines a number of presently to be had advertisement structures to spotlight either strengths and components for improvement.

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Until monoclonal antibodies became commercially available in 1981, it was extremely difficult to measure immunofluorescence from samples treated with more than one antibody. Although monoclonal antibodies made it relatively simple to stain with multiple antibodies, there were, initially, no combinations of antibody labels with well- separated fluorescence emission compatible with 488 nm excitation. The short-term solution to this problem required the addition of a second laser, either a water-cooled krypton ion laser or a dye laser, to the instrument, making it substantially more complex, power-hungry, water-thirsty, and expensive.

19] G. N. Papanicolaou and H. F. Traut, The diagnostic value of vaginal smears in carcinoma of the uterus. Amer. J. Obstet. Gynecol. 42, 193–206 (1941). [20] T. O. Caspersson, Cell Growth and Cell Function. Norton: New York, NY (1950). [21] R. C. Mellors and R. Silver, A microfluorometric scanner for the differential detection of cells: application to exfoliative cytology. Science 114, 356–360 (1951). [22] W. E. Tolles, The Cytoanalyzer: An example of physics in medical research. Trans. Y. Acad.

2] H. M. Shapiro, The evolution of cytometers. Cytometry 58A, 13–20 (2004). [3] H. M. Shapiro, Cytometry and cytometers: Development and growth. , Eds. Flow Cytometry with Plant Cells. Weinheim, Wiley-VCH: Weinheim, Germany, pp. 1–17 (2007). [4] H. Harris, The Birth of the Cell. Yale University Press: New Haven, CT, pp. 1–212 (1999). 75in References [5] R. Hooke, Micrographia. John Martyn & James Allestry, Printers to the Royal Society: London, England, 1665, pp. 1-246. ) [6] T. Cowles, Dr. Henry Power’s poem on the microscope.

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The Microflow Cytometer by Frances S. Ligler, Jason S. Kim

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