By Gail Cardew, Jamie A. Goode,
Brings jointly a cross-fertilization of principles among human molecular genetics, develeopmental biology, tissue biology and the biochemistry of mobilephone signalling pathways, so one can create new insights into the mechanisms of standard and irregular skeletogenesis.
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Additional resources for The Molecular Basis of Skeletogenesis No. 232
Antisense probes were generated as described: BmpRIB and colII (Francis-West et al 1999), and sox9 (Kent et al 1996). Histology BrdU incorporation and detection, wholemount nile blue staining, alcian blue staining on whole embryos or on sections, were executed as in (Zou et al 1997), TUNEL as in (Chen et al 1994) and PNA staining as in (Stringa & Tuan 1996). Results Noggin misexpression disrupts chondragenesis Widespread Noggin misexpression in the limbs before overt chondrogenesis (infection from stage 14 to 20) produced very short limbs completely lacking alcian blue-stained cartilage (Fig.
Histology BrdU incorporation and detection, wholemount nile blue staining, alcian blue staining on whole embryos or on sections, were executed as in (Zou et al 1997), TUNEL as in (Chen et al 1994) and PNA staining as in (Stringa & Tuan 1996). Results Noggin misexpression disrupts chondragenesis Widespread Noggin misexpression in the limbs before overt chondrogenesis (infection from stage 14 to 20) produced very short limbs completely lacking alcian blue-stained cartilage (Fig. 1A, n=29/30, assayed up to stage 31).
It seems to be almost constitutively expressed in early mesenchyme, and then it comes up a little bit more in the condensing regions. Unlike the proteoglycan core protein, for example, which seems to be stringently regulated, with no sign of the mRNA until differentiation is about to happen, type II collagen is expressed much more broadly and much earlier (Kosher et al 1986a,b). Hall: Including in places such as basal membranes where you normally would not expect to find type II collagen. Sandrine Pizette, have you had any of your embryos go much longer to see whether those cells that express collagen type II do anything?
The Molecular Basis of Skeletogenesis No. 232 by Gail Cardew, Jamie A. Goode,