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Sohn, Bonn. , LEWIS L. , and BUMPUS F . M. (1958) Separation and characterization of the protein moiety of human a 1 -lipoprotein. Arch. Biochem. Biophys. 74, 390-397. SCHMID K. (1950) Preparation and properties of an acid glycoprotein prepared from human plasma. / . Am. Chem. Soc. 72, 2816. SCHWIMMER S. and BALLS A. K. (1949) Starches and their derivatives as adsorbents for malt a-anylase. / . Biol. Chem. 180, 883-894. SCOTT D . A. (1934) Crystalline insulin. Biochem. J. 28, 1592-1602. STUMPF P.

The tube is evacuated, filled with 02-free N 2 , and re-evacuated. The whole tube is immersed in the water bath at 37°, and after 2 min, the contents are mixed. The time from the moment of mixing to a complete decoloration of méthylène blue is measured with a stop-watch. When the decoloration time is between 10 sec and 60 sec, the time is nearly proportional to the amount of the enzyme used. This measurement is effective only when no reduction of méthylène blue occurs in the absence of lactate.

Academic Press, New York. Muus J. (1953) Studies on salivary amylase with special reference to the interaction with chloride ions. Compt. Rend. , Carlsberg 28, 317-334. SEPARATION OF PROTEINS 29 NoRTHROP J. H . (1946) Crystallization of pepsin from alcohol. / . Gen. Physiol. 30, 177184. ONCLEY J. , GURD F . R. , and MELIN M. (1950) Preparation and properties of serum and plasma proteins. XXV. -lipoproteins. / . Amer. Chem. Soc. 72, 458-464. ORD M. G. and THOMPSON R. H . S. (1951) The preparation of soluble cholinesterases from mammalian heart and brain.

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The Separation and Isolation of Proteins. A Laboratory Manual of Analytical Methods of Protein Chemistry (Including Polypeptides)


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