By Gaylord D. Paulson
content material: Xenobiotic metabolism in vegetation : in vitro tissue, organ, and remoted mobilephone thoughts / R.H. Shimabukuro and W.C. Walsh --
Xenobiotic metabolism in better vegetation : in vitro tissue and cellphone tradition strategies / Ralph O. Mumma and Robert H. Hamilton --
Pesticide metabolism in better vegetation : in vitro enzyme reviews / G.L. Lamoureux and D.S. Frear --
innovations for learning the metabolism of xenobiotics via intact animal cells, tissues, and organs in vitro / Robert E. Menzer --
using animal subcellular fractions to check sort I metabolism of xenobiotics / Ronald W. Estabrook, Jurgen Werringloer, and Julian A. Peterson --
using animal subcellular fractions to review style II metabolism of xenobiotics / Maureen J. Crawford and David H. Hutson --
tools for the learn of metabolism of xenobiotics in insect mobile and organ cultures / Edwin P. Marks, Malcolm J. Thompson, and William E. Robbins --
using insect subcellular elements for learning the metabolism of xenobiotics / C.F. Wilkinson --
using in vitro recommendations to check the comparative metabolism of xenobiotics / L.C. Terriere.
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Extra info for Xenobiotic Metabolism: In Vitro Methods
Agric. Food Chem. (1971) 19, 1183. 61. , Stephenson, G. R. and Smith, L. , J. Agric. Food Chem. (1971) 19, 1187. 62. Hamill, A. S. , Weed Sci. (1973) 21, 330. 63. Hamill, A. S. , Weed Sci. (1973) 21, 335. 64. Hamill, A. S. , Weed Sci. (1973) 21, 339. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1979. 1. SHIMABUKURO AND WALSH Plant Tissues, Organs, and Isolated Cells 65. Lamoureux, G. L . , Stafford, L. , Shimabukuro, R. H. and Zaylskie, R. , J. Agric. Food Chem. (1973) 21, 1020.
The c a l l u s growth and i t s n u t r i e n t s t a t u s was not changed. The metabolites found i n the p l a n t s a r e a l s o present i n the c a l l u s t i s s u e , but differ quantitatively. Of p a r t i c u l a r note i s the f a c t that f r e e hydroxylated 2,4-D e x i s t s i n both c a l l u s and i n p l a n t s i n c o n t r a s t to e a r l i e r t i s s u e c u l t u r e experiments. Free hydroxylated 2,4-D a l s o has been reported i n bean p l a n t s (48). These experiments a l s o suggest that the unknown compounds, Unki and U n k 2 , a r e amino a c i d conjugates o f hydroxylated 2,4-D metabolites ( p r i m a r i l y the glutamic a c i d conjugate of 4-OH-2,5-D) and are common to a l l t i s sue examined.
Whole plants have and "terminal" residue using isolated leaves or roots, leaf discs or sections, and separated mesophyll cells have proven to be more useful for short-term investigations on xenobiotic metabolism, mechanism of action and selectivity. The in vitro techniques allow the treatment of large amounts of plant material with a minimum of chemical and facilitate rapid uptake of the chemical into plant tissues. The influence of root and leaf surface absorption is also circumvented by the use of in vitro techniques.
Xenobiotic Metabolism: In Vitro Methods by Gaylord D. Paulson